Cell Biolabs? Aflatoxin DNA Adduct Competitive ELISA Kit provides a convenient method for the detection of total Aflatoxin B1-DNA adducts (ring-opened and ring-closed forms). First, the unknown AFB1-DNA samples or AFB1-DNA standards are added to the AFB1-DNA conjugate preabsorbed ELISA plate. After a brief incubation, an anti-AFB1-DNA antibody is added, followed by an HRP conjugated secondary antibody. The total content of AFB1-DNA in unknown samples is determined by comparison with a predetermined AFB1-DNA standard curve.
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