Monkeypox Virus Real Time PCR Kit

The Bioperfectus Monkeypox Virus Real Time PCRT Kit is based on real-time PCR technology. Specific primers and probes are designed based on specific areas of Monkeypox virus. Probes consist of a reporter dye at 5' and quenching dye at 3'. The fluorescent signals emitted from reporter dye are absorbed by the quencher, so it doesnt's emit signals. During amplification, probes bonded to templates are cut off by Taq enzyme (5'->3' exonuclase activity), separating reporter dye from the quencher, generating fluorescent signals, the PRC instrument will then automatically draw a real-time amplification curve based on the signal change, finally realizing the qualitative detection and differentiation of DNA from Monkeypox virus. In addition, the kit is also contains a housekeeping gene (RNase P) as an internal control (IC) for specimen sampling and nucleic acid extraction.